sp2 mil6 mouse myeloma cells (ATCC)

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ATCC sp2 mil6 mouse myeloma cells
Sp2 Mil6 Mouse Myeloma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 981 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sp2+mil6+mouse+myeloma+cells/Sp2%2FmIL-6/pmc11539575-152-11-15
Average 96 stars, based on 981 article reviews

sp2 mil6 mouse myeloma cells - by Bioz Stars, 2026-10

96/100 stars

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Purification:

Article Title: Mechanisms and inhibition of Porcupine-mediated Wnt acylation.
Article Snippet: Wnt signalling is essential for regulation of embryonic development and adult tissue homeostasis, and aberrant Wnt signalling is frequently associated with cancers.. Wnt signalling requires palmitoleoylation on a hairpin 2 motif by the endoplasmic reticulum-resident membrane-bound O-acyltransferase Porcupine (PORCN).. This modification is indispensable for Wnt binding to its receptor Frizzled, which triggers signalling.

Generated:

Article Title: Development of a monoclonal antibody to study MARCH6, an E3 ligase that regulates proteins that control lipid homeostasis.
Article Snippet: .. Hybridoma cells were generated by fusing the isolated splenic lymphocytes with SP2-mIL6 mouse myeloma cells (ATCC, Cat# CRL-2016) by electro-cell fusion (ECFG21 Nepagene Super Electro-cell Fusion generator). .. The fused hybridoma cells were set up in Clonacell-Hy medium C (Stemcell Technologies, Cat# 03803) supplemented with 1xHAT (Gibco, Cat# 21060-017), which selects for properly fused cells.

Article Title: Development of a monoclonal antibody to study MARCH6, an E3 ligase that regulates proteins that control lipid homeostasis
Article Snippet: .. Hybridoma cells were generated by fusing the isolated splenic lymphocytes with SP2-mIL6 mouse myeloma cells (ATCC, Cat# CRL-2016) by electro-cell fusion (ECFG21 Nepagene Super Electro-cell Fusion generator). .. The fused hybridoma cells were set up in Clonacell-Hy medium C (Stemcell Technologies, Cat# 03803) supplemented with 1x hypoxanthine-aminopterin-thymidine (HAT; Gibco, Cat# 21060-017), which selects for properly fused cells.

Isolation:

Mouse Assay:

Article Title: Structure and inhibition of the human lysosomal transporter Sialin
Article Snippet: .. IgG-10B8, a mouse monoclonal anti-human Sialin antibody, was prepared by fusion of SP2-mIL6 mouse myeloma cells (ATCC no. CRL-2016) with splenic B lymphocytes obtained from BALB/c mice ( n = 1) at UT Southwestern with the approval of the Institutional Animal Care and Research Advisory Committee #2017-102391 as previously described . .. Briefly, mice were immunized with one primary and four boosts of purified recombinant human Sialin reconstituted with liposomes in phosphate buffered saline (PBS) combined with Sigma Adjuvant System.

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